The Expression Of Matriptase Centered On Lung Cancer Cells

The Expression of Matriptase centered networking proteins in Lung Cancer Cells Runyu Zhong1 Chen-yong Lin2 Michael D Johnson2 1Department of Biochemistry Molecular and Cellular Biology and 2Lombardi Cance Center, Georgetown University Medical Center, 3900 Reservoir Rd., NW, Washington DC, 20057-1455 Abstract Pulmonary carcinoma, also known as Lung Cancer, in among the most prevalent and thermal kinds of cancers in the world. A network around Matriptase including it s inhibitor HAI-1, HAI-2 and it s downstream substrate prostasin play a significant role in both the epithelia construction in normal epithelial cells and tumor development and progression in cancer cells. My research focuses on the expression of matriptase, HAI-1, HAI-2 and prostasin in lung cancer cell line NCI-H209, NCI-H292 and NCI-H1944 by means of Western blot and Inmunoflurescense. Antibodies M23, M19 and DC16 are used to recognize matriptase, HAI-1 and HAI-2, respectively and antibidies YL89, YL11 and YL10 are used to recognize prostasin in both experiment. All the 4 proteins in the network are detected to express in all cells line used in research and they express both on cells menbrane and inside cells in NCI-H1944. Both HAI-1 and HAI-2 can act as inhibitors to both matriptase and prostasin while HAI-1 is th e predominant inhibitor. This network should be very essential to the oncogenesis and metastasis of lung cancer cells. Introduction Pericellular proteolysis plays important roles in many